Blood-Brain Barrier Built on a Chip

Vanderbilt-led team developed ‘brain on a chip’

 

A $2.1M “microbrain” challenge for the purpose of studying chemical reactions in the brain was announced in July, 2014. The research grant was awarded to researchers from Vanderbilt University, Vanderbilt University Medical Center, the Cleveland Clinic and Meharry Medical College. According to Vanderbilt, the grant was one of 17 issued “by the National Center for Advancing Translational Sciences at the National Institutes of Health as part of a $70 million ‘Tissue Chip for Drug Testing’ program. The five-year program is a cooperative effort on the part of NIH, the Defense Advanced Research Projects Agency and the FDA.”

Photo: Courtesy of Vanderbilt University. Top = Brain Cells, Bottom = Bioengineered Vessels. Membrane Between = Blood-Brain Barrier.

Why is the blood-brain barrier an important challenge? 

The are many “organs-on-a-chip”  being microfabricated  (see this list called Meet Chip through National Institutes of Health) in order to speed up and improve the pace of research and development particularly for drugs, which are currently tested with cell cultures and animals or humans. The intention is to reduce the time, cost, and technical difficulty of drug development including rapid detection of side effects or lack of effectivity. In addition, it can aid in the understanding of diet and disease in similar ways.

The brain has been historically a challenge for developing drugs or medical therapies because it is surrounded by three highly selective semipermeable membrane barriers that separate blood from brain extracellular fluid (ECF). These barriers block molecules. According to ABC Science: “About 98 per cent of today’s medications cannot cross the blood-brain barrier in significant quantities. Mind you, drugs such as most antipsychotics, sleeping aids and antidepressants are smaller than 500 daltons and can sneak through. But they’re in the two per cent of drugs that can get through.”

How was the Neuro Vascular Unit (NVU) On -A-Chip built? 

The official name of the device is NeuroVascular Unit (NVU) on a chip. The top of the chip is the brain simulation created from stem cells (IPSC’s) to generate the simulated tissues (i.e. brain) and the bottom side simulates blood vessels. The microfluid system transports liquid across the membrane using micropumps that can sample and deliver chemicals/fluids including cerebral spinal fluid. Human endothelial cells line up along the “blood side” in a matter of days and mimic the blood-brain barrier. Cells are also added to the “brain side” to mimic that end of the blood-brain barrier including astrocytes, pericytes, and excitatory neurons.

 

Photo: Courtesy of Vanderbilt University. Blue Dye = Brain Cells, Red Dye = Blood Circulation

According to Research News @ Vanderbilt University: 

https://news.vanderbilt.edu/2012/07/24/microbrain/

“Given the differences in cellular biology in the brains of rodents and humans, development of a brain model that contains neurons and all three barriers between blood, brain and cerebral spinal fluid, using entirely human cells, will represent a fundamental advance in and of itself,” said John Wikswo, the Gordon A. Cain University Professor and director of the Vanderbilt Institute for Integrative Biosystems Research and Education (VIIBRE), who is orchestrating the multidisciplinary effort.

“Wikswo and his collaborators argue that this new type of brain model should provide new insights into how the brain receives, modifies and is affected by drugs and disease agents. By replicating the forms of chemical communication and molecular trafficking that take place in the human brain, the device will allow them to test the effectiveness of various drug and nutritional therapies designed to prevent both acute injuries like strokes and chronic diseases like obesity and epilepsy, as well as uncovering the potential adverse effects of experimental drugs.”

Photo: Courtesy of Vanderbilt University

Photo: Courtesy of Vanderbilt University

Related News: 

  1. Vanderbilt UniversityVanderbilt-led team to develop ‘microbrain’ to improve drug testing
  2. NIHBlood-Brain Barrier on a Chip 
  3. Medgadget. Scientists Build Blood-Brain Barrier On-a-Chip to Help Develop Neuro Drugs, Understand Brain Diseases

 

Gif: Courtesty of mediagiphy.com

– Keri Kukral, @kerikukral

 

Video: Courtesy of YouTube, Vanderbilt University 

 

Subscribe@Raw Science TV |Facebook@Raw Science |Twitter@RawScienceTV |YouTube@RawScience1

 

 

 

13 Comments

  • Reply February 3, 2017

    Lowongan Kerja

    it was very nice.

  • Reply February 9, 2017

    robert flower

    God bless Dr. sebi for his marvelous work in my life, I was diagnosed of HERPES since 2014 and I was taking my medications, I wasn’t satisfied i needed to get the HERPES out of my system, I searched about some possible cure for HERPES i saw a comment about Dr. sebi, how he cured HERPES DIABETES and CANCER with his herbal medicine, I contacted him and he guided me. I asked for solutions, he started the remedy for my health, he sent me the medicine through UPS SPEED POST. I took the medicine as prescribed by him and 14 days later i was cured from HERPES, Dr. sebi truly you are great, do you need his help also? Why don’t you contact him through dr.sebitradomedical@gmail.com

  • Reply February 19, 2017

    motu patlu

    … [Trackback]

    […] Read More: rawscience.tv/blood-brain-barrier-built-on-a-chip/ […]

  • Reply February 24, 2017

    number clicker game

    … [Trackback]

    […] Read More Infos here: rawscience.tv/blood-brain-barrier-built-on-a-chip/ […]

  • Reply February 24, 2017

    subway surf

    … [Trackback]

    […] There you will find 70698 more Infos: rawscience.tv/blood-brain-barrier-built-on-a-chip/ […]

  • Reply February 24, 2017

    hampton bay led lights

    … [Trackback]

    […] Find More Informations here: rawscience.tv/blood-brain-barrier-built-on-a-chip/ […]

  • … [Trackback]

    […] Informations on that Topic: rawscience.tv/blood-brain-barrier-built-on-a-chip/ […]

  • Reply March 8, 2017

    kimberly

    Hello everyone, this is serious. My Brother of 39 years who has suffered of HERPES for 1 and half years has been cured by a Herbal doctor called Dr. KEN who uses herbal and traditional medicine to cure people. I have spent so much on my Brother’s health paying hospital bills for years but no improvement. A friend told me about this herbal man who cure people with herbal medicine. He cures all sickness, diseases, viruses, and infections with his different herbal curing medicine.
    The result that declares my Brother Roland healed was from a hospital here in New York, And the results of the test says my brother was okay. Dr KEN directed that my Brother would be okay in 21 days if he uses the medicine as directed. And 21 days later, we went to confirm it from the central hospital and several test that was done says my brother is fully healed. So he was cured by this herbal doctor in 21 days. This amaze me though. I would advice everyone to meet this Dr KEN who made my Brother healthy again.
    Meet Dr KEN and set yourself and your love ones free.
    Doctor KEN’s email again is kenherbalcure@gmail.com call or whats app +2347034971061

  • Reply March 8, 2017

    anal

    … [Trackback]

    […] Read More: rawscience.tv/blood-brain-barrier-built-on-a-chip/ […]

  • Reply March 9, 2017

    Firstrow

    … [Trackback]

    […] Read More: rawscience.tv/blood-brain-barrier-built-on-a-chip/ […]

  • Reply March 11, 2017

    joker hack

    … [Trackback]

    […] Read More here: rawscience.tv/blood-brain-barrier-built-on-a-chip/ […]

  • … [Trackback]

    […] Read More: rawscience.tv/blood-brain-barrier-built-on-a-chip/ […]

  • … [Trackback]

    […] Informations on that Topic: rawscience.tv/blood-brain-barrier-built-on-a-chip/ […]

Leave a Reply